Custom synthesized Phos-tag™ compounds
We synthesize special Phos-tag™ compounds.
Phos-tag™ is a functional molecule that traps every phosphoprotein, including Ser, Thr, and Tyr. We have a series of products available for the separation, detection, analysis, and purification of phosphoprotein.
List of customized Phos-tag™ products
We offer special sales of the customized Phos-tag™ products in stock.
Phos-tag™ Click beads™ KIT
Phos-tag™ Click Tip™ KIT
This kit provides a rapid, sensitive, and non-toxic method for the enrichment of azide-containing molecules.
Simple process using Phos-tag™ Agarose
Small starting amounts(100µg~)
No additional reagents during SPAAC(or SPIEDAC)reaction
Direct Detection of azide-labeled peputides
Phos-tag™ Click beads™ and Phos-tag™ Click Tip™ was jointly developed with the Advanced Medical Research Center of Yokohama City University and NARD Inst., Ltd.
Azido-labeled Protein Purification / Concentration Kit
Phos-tag™ Click beads™ Kit 01
| nCB-K01 |
|---|
| pBCN Ligand 1mg(3µmol) Phos-tag™ Agarose 90µL-gel(180µL slurry) |
| 32,000 JPY (35,200 JPY include consumption tax) |
Phos-tag™ Click Tip™ Kit 02
| nCT-K02 |
|---|
| pBCN Ligand 1mg(3µmol) Phos-tag™ Agarose 90µL-gel(180µL slurry) C18 pipette tip 6 pieces |
| 32,600 JPY (35,860 JPY include consumption tax) |
Phos-tag™ fluorescent stain gel
This is a reagent for phosphorylation fluorescent imaging analysis that allows for staining at physiological pH. The reagent selectively binds with the pSer, pThr, pTyr, pHis, and pAsp residues. The staining process requires approximately 2 hours. Radioactive materials, chemical labels, or antibodies are not required. Procedures lower in the procedural stream, such as CBB staining or MS analyses, can be applied.
Visualization is also possible by combining Phos-tag™ SDS–PAGE and conventional SDS–PAGE.
Phos-tag™ Yellow
| nPGS-Y01 |
|---|
| Ex/Em=505nm/514nm |
| 0.2mg 20,000 JPY (22,000 JPY include consumption tax) |
Phos-tag™ Magenta
| nPGS-M01 |
|---|
| Ex/Em=547nm/561nm |
| 0.2mg 20,000 JPY (22,000 JPY include consumption tax) |
Phos-tag™ Cyan
| nPGS-C01 |
|---|
| Ex/Em=643nm/661nm |
| 0.2mg 20,000 JPY (22,000 JPY include consumption tax) |
Phos-tag™ Aqua
| nPGS-A01 |
|---|
| Ex/Em=551nm/564nm |
| 0.2mg 20,000 JPY (22,000 JPY include consumption tax) |
Mixed reagents for Phos-tag™ Common Solution 5x
| nPGS-MR1 |
|---|
| A stain solution/washing solution reagent is available for Phos-tag™ fluorescent stain gels. A common solution of 5× concentration is made by diluting with purified water to 500 ml. |
| 5,000 JPY (5,500 JPY include consumption tax) |
Any product contains reagents that can be used for staining a mini-gel at least 20 times.
Phos-tag™ for condensing phosphorylated peptide
Phos-tag™ MG-Bead
Phos-tag™ ligand carrying no magnetic beads, allowing high throughput for experiments.
Binding volume: 1µmol/mL-gel
| nMG-303 |
|---|
| 100µL-gel 99,000 JPY (108,900 JPY include consumption tax) |
Phos-tag™ Tip
Product grade is optimized for the use of mass analysis.
| nAG1-103 [High-sensitive MS analysis] |
|---|
| 10µL-gel x8 18,000 JPY (19,800 JPY include consumption tax) |
Production-to-order products
We synthesize your requested Phos-tag™ tip on a made-to-order basis.
| nAG2-103 [Large volume] |
|---|
| 50µL-gel x8 64,000 JPY (70,400 JPY include consumption tax) |
Phos-tag™ labeled with a dye
This is a dye-labeled Phos-tag™ ligand.
Phos-tag™ Coumarin
| nFLC-301 |
|---|
| 10mg 49,000 JPY (53,900 JPY include consumption tax) |
Phos-tag™ Dabsyl
| nFLD-303 |
|---|
| 10mg 49,000 JPY (53,900 JPY include consumption tax) |
Amino acid Phos-tag™
Complex of Phos-tag™ and amino acid. The N-end is protected by Fmoc.
Phos-tag™ Lys
| nAML-201 |
|---|
| 10mg Pricing can be separately discussed |
Phos-tag™ Glu
Production-to-order products
| nAMG-202 |
|---|
| 10mg Pricing can be separately discussed |
References
“A Phos-tag™-based magnetic-bead mehthod for rapid and selective separation of phosphorylated biomolecules”
Journal of Chromatography B, 925, 86-94 (2013)
M. Tsunehiro, Y, Meki, K. Matsuoka, E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike
“A Phos-tag™-based Fluorescence Quenching System for Activity Assay and Inhibitor Screening for Alkaline Phosphatase”
American Journal of Analytical Chemistry, 5, 796-804 (2014)
E. Kinoshita-Kikuta, H. Kurosaki, N. Kunisada, E. Kinoshita, and T. Koike
“A Phos-tag™-based fluorescence resonance energy transfer system for the analysis of the kinase reaction of a substrate peptide”
Analytical Methods, 3,1303-09 (2011)
M. Somura, K. Takiyama, E. Kinoshita-Kikuta, E. Kinoshita and T. Koike
“Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus”
Invertebrate Survival Journal, 11,213-218 (2014)
Y. Sogame, K. Kojima, T. Takeshita, E. Kinoshita and T. Matsuoka
“Phos-tag™ beads as an immunoblotting enhancer for selective detection ofphosphoproteins in cell lysates” Analytical Biochemistry, 389,83-85 (2009) E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike
In addition to available Phos-tag™ products,
we handle the synthesis of customized Phos-tag™ compounds required for your research.
Please let us know your request.
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